NA0105_Mouse Anti His Tag Antibody, mAb

Mouse Anti His Tag Antibody, mAb

Cat: NA0105

Name: Mouse Anti His Tag Antibody, mAb

Immunogen: HHHHHH peptide conjugated to keyhole limpet haemocyanin(KLH)

Host Species: Mouse

Specificity: This antibody recognizes C-terminal, N-terminal, and internal region of His tag fusion proteins.

Form: Liquid

Concentration: 0.5 mg/ml

Storage Buffer: 1×PBS, pH7.4, containing 0.1% BSA, 50% glycerol and 0.03% Proclin 300.

Purification: Purified by Protein A

Clonality: Monoclonal

Isotype: Mouse IgG1, κ

Clone ID: 90B8

Shipping and Storage: Shipped in ice bag. The antibody can be stored for 1-2 weeks at 2-8°C. For long term storage, store it at -20°C. Avoid repeated freezing and thawing cycles.

Expiration Date: Specified on product label.

Applications

Application	Recommended Usage
ELISA	0.05-0.2µg/ml
Western Blot	0.1-1µg/ml
IP	1µg/ml
Other applications	User-optimized

Note: Ningyang Biotech can offer this product according to your requirement, including product size, buffer components, etc.

Examples

Figure 1. Detection of C- or N-terminal His tags in recombinant fusion proteins were analyzed by Western blot using Mouse Anti His Tag Antibody, mAb (NingyangBiotech,Cat.NA0105, at 1 µg/mL concentration).

The signal was developed with Goat Anti-Mouse IgG(H&L)[HRP], pAb(NingyangBiotech Cat.NA0035).

The results show that Mouse Anti His Tag Antibody, mAb (NingyangBiotech, NA0105) can recognize C-terminal and N-terminal His-tagged proteins.

Figure 2. 5×His was detected in HHHHH tagged fusion protein by Western blot using Mouse Anti His Tag Antibody, mAb (NingyangBiotech, Cat.NA0105).

Figure 3. Detection of His tag recombinant fusion proteins were analyzed by Western blot using Mouse Anti His Tag Antibody, mAb (NingyangBiotech, Cat.NA0105, at 0.2 µg/mL concentration).

The signal was developed with Goat Anti-Mouse IgG(H&L)[HRP], pAb(NingyangBiotech, Cat.NA0035).

 

For research use only. Not intended for human and animal therapeutic or diagnostic use.

 

Mouse Anti His Tag Antibody, mAb_Cat.NA0105_Datasheet